

Back to top
|
Ask
to top
Ask
YSI 2900 Biochemistry Analyser
The YSI biochemistry analyser is recognized as the gold standard in bio analytical instruments with highly accurate sensors and rapid results. This instrument uses an enzyme sensor to achieve analyte specific results in seconds. The specificity of the enzyme allows highly accurate results without interference as in various spectrophotometric methods. The unique fluidics and chamber resist clogging even at high cell densities. The equipment has an intuitive graphical interface and touch screen display
VIDEO
YSI biochemistry analyzer
FREQUENTLY ASKED QUESTIONS
................................................................................................................................................
................................................
How does the YSI technology work?
Immobilized Enzyme Biosensor
YSI biosensor technology is the key to fast, accurate measurement.
An enzyme specific for the substrate of interest is immobilized between two membrane layers, polycarbonate and cellulose acetate. The substrate is oxidized as it enters the enzyme layer, producing hydrogen peroxide, which passes through cellulose acetate to a platinum electrode where the hydrogen peroxide is oxidized. The resulting current is proportional to the concentration of the substrate.
YSI membranes contain three layers. The first layer, porous polycarbonate, limits the diffusion of the substrate into the second layer (enzyme), preventing the reaction from becoming enzyme-limited. The third layer, cellulose acetate, permits only small molecules, such as hydrogen peroxide, to reach the electrode, eliminating many electrochemically-active compounds that could interfere with the measurement.
Close X
How does the YSI results compare with the results from HPLC?
• Breakfast Cereals: YSI analyzer vs. HPLC
The sucrose and dextrose concentrations of 9 breakfast cereals correlated well when measured with a YSI analyzer and HPLC.
|
YSI Analyzer % w/w |
HPLC % w/w |
|
||
Sample |
Sucrose |
Dextrose |
Sucrose |
Dextrose |
Label* |
A |
35.0 |
1.24 |
36.0 |
0.96 |
38.8 |
B |
21.4 |
0.85 |
22.2 |
0.50 |
21.1 |
C |
22.4 |
1.52 |
23.1 |
1.52 |
24.6 |
D |
5.4 |
1.03 |
6.7 |
0.70 |
7.0 |
E |
17.4 |
0.27 |
18.3 |
0.07 |
28.0 |
F |
25.2 |
1.61 |
25.4 |
1.33 |
28.2 |
G |
29.8 |
3.51 |
27.9 |
4.14 |
35.2 |
H |
32.2 |
3.0 |
31.1 |
2.42 |
38.7 |
I |
32.9 |
0.71 |
30.4 |
0.43 |
38.7 |
*Label information reported as sucrose and other sugars.
• Glutamate: Spike and recovery
• To evaluate the accuracy of YSI's glutamate measurement, each sample was spiked with a known concentration of glutamate. In each case, nearly 100% of the spike was recovered indicating no significant matrix interferences.
Product |
Recovery |
Samples |
Chicken broth |
100.1% |
6 |
Salad dressing |
100.0% |
3 |
Chow mein |
99.7% |
3 |
Spice mix |
99.0% |
3 |
Tomatoes |
99.7% |
3 |
• Choline: Spike and recovery
• To evaluate the accuracy of YSI's choline measurement, each sample was spiked with a known concentration of choline. In each case, nearly 100% of the spike was recovered indicating no significant matrix interferences.
Product |
Spike |
Recovery |
Infant formula |
12% |
105% |
35% |
98.9% |
|
60% |
98.6% |
|
Infant formula with protein hydrolysate |
50% |
99.1% |
65% |
96.1% |
|
75% |
96.4% |
|
100% |
96.8% |
• Glutamine in Cell Culture: YSI analyzer vs. HPLC
• The glutamine concentration of a cell culture was measured with a YSI analyzer and with HPLC. The results correlated well but, unlike HPLC, the YSI results were available immediately for process adjustments.
Close X
Who do I go to when the instrument breaks down?
Arasains has the support staff to do the service of the machine as well as support staff to answer your chemistry problems
Close X
For more information on the above tests contact the friendly Arasains sales staff or fill in the enquiry form.
For more questions ask Dr Lee
Caution : not all question can or will be answered