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Mycotoxin and Shell Fish Toxin Detection
Mycotoxins are toxic chemical compounds produced by molds. There are several mycotoxins that has become a concern in industry; Aflatoxin B1, B2, G1, G2; Aflatoxin M1; Fumonisisn B1, B2, B3; Zearalenone; Ochratoxin A; etc. These mycotoxins can affect humans and animals because they are carcinogenic. PT. Arasains offer several kits that can help to detect mycotoxins using lateral flow (RIDA®QUICK), ELISA method (RIDASCREEN®) and immunoaffinity column (IAC) for HPLC.
International standards and code of practice for mycotoxins are established by codex allimentarius (www.fao.org/fileadmin/user_upload/agns/pdf/CXS_193e.pdf). In the EU, the standards for mycotoxins are listed in the commission regulation EC No. 1881/2006 (eur-lex.europa.eu/legal-content/EN/TXT/). In Indonesia, the standards for mycotoxins in food are listed in the BPOM regulation No. 8 Tahun 2018 (standarpangan.pom.go.id/dokumen/peraturan/2018/Salinan_PerBPOM_8_Tahun_2018_tentang_Cemaran_Kimia_Pangan_Join.pdf) and for feed are listed in the SNI 4483:2013.
High concentrations of shell fish toxins can accumulate in many marine filter feeders such as clams, mussels and oysters. There are four syndromes are usually caused by shell fish poisoning ie paralytic shell fish poisoning (PSP), diarrhetic shell fish poisoning (DSP), neurotic shell fish poisoning (NSP) and amnesic shell fish poisoning (ASP). The polyether toxin; okadiac acid and its analogues ie dinophysistoxin DTX1, DTX2, DTX3 causes DSP.
The EU regulation EC 385/2004 (eur-lex.europa.eu/LexUriServ/LexUriServ.do) stated that lives mollusks must not contain okadaic acid and/or its equivalents like dinophysistoxin and pectenotoxins of more than 160 ug per kilo. The EU regulation (EC) No. 853/2004 states that mollusks must not contain PSP toxin (saxi toxin and its analogues) of more than 800 ug per kg. In the European Union, Regulation (EC) No. 853/2004 (eur-lex.europa.eu/LexUriServ/LexUriServ.do) stipulates that live bivalve mollusks must not contain PSP toxins (saxi toxin and analogues) in total quantity (measured in the whole body or any part edible separately) that exceeds a limit of 800 ug/ kg
Lateral Flow Strips for Mycotoxin Principles of Test
Lateral flow strips are easy to use and require little equipment. A sample is placed onto the sample pad. The sample diluent in the sample flow upwards through capillary action past the conjugate pad. Here the antigen ie the mycotoxin picks up a colloidal conjugate and carries it towards the test line where antibody bound onto nitrocellulose strip will pick up the antigen bound conjugate. The conjugate is colored and show up as a colored line. Conjugate that do not pick up any antigen will be carried past the test line to the control line where it will show up as another colored line. Positive samples will have two colored lines and negative one line at the control position.
RIDA®QUICK Lateral Flow Strips for Mycotoxin
- Rapid result
- Simple sample prepration and assay procedure
- Allow to on-site decision about mycotoxin contamination
- Option for semi-quantitative/fully-quantitative determination
The RIDA®SMART APP allows for quantitative measurements of mycotoxins with the use of lateral flow strips. This app is installed into a smartphone and scanning the strips with the smart phone camera allows the color intensity to be correlated to concentration of the mycotoxin. The app provides reliable and accurate results, which can be forwarded via e-mail, exported to a cloud server or easily sent to any supported printer.
RIDASCREEN® Microtiter Plate ELISA Test for Mycotoxin
RIDASCREEN® ELISA test for mycotoxin are based on competitive immunoassay. The base of the microtitier plate are coated with antibodies anti to the mycotoxin selected. Sample containing the antigen are added together with an antigen conjugate. The antigen competes with the conjugate bound antigen bound conjugate for a place on coated plate. A color substrate is then added to the well and its color strength will depend on the amount of mycotoxin. The more mycotoxin means that the color will less intense giving a curve with a negative gradient. The wells are read with a microtiter plate reader.
RIDASCREEN® ELISA Test for Mycotoxin
Uses the high specificity of antigen and antibody interaction to detect and quantify mycotoxins by photometric measurement. Final analysis is carried out with RIDA®SOFT Win.
- Quantitative result
- High sensitivity and specificity
- Fast and reliable
- Able to screen high samples numbers
- Walk away automation is available with Chemwell and Thunderbolt machine
Uses the high specificity of antigen and antibody interaction to isolate, purify and concentrate mycotoxins in a wide range of commodities in conjunction with HPLC or LC-MS/MS.
- Multi analysis in conjunction with HPLC and LC-MS/MS
- Reduce background interference for improve accuracy of results
- Multiple applications
- Available in 1 mL and 3 mL formats
- Aflatoxin Total
- Aflatoxin M
- Trichotecene P
- T-2 & HT-2
- Ochratoxin A
- Multitoxin DON, Zearalenone, T-2 and HT-2
- Multitoxin for Aflatoxin and Ochratoxin
- Multitoxin for Aflatoxin, Ochratoxin, Fumonisin
- Multitoxin for Aflatoxin, Ochratoxin, Zearalenone
R-biopharm Mycotoxin Cards for Qualitative Analysis
This is a qualitative screening card for the detection of mycotoxins at various levels. The kit is based on monoclonal antibody detection technology on a card format. The toxin is extracted from the sample filtered and passed through the immunoaffinity column before being diluted and added to the card. A conjugate is added to the card and the unbound conjugate is then removed by washing. A colorless substrate is added and the card is incubated for five minutes. A stop solution is finally added and results interpreted as below
For more information on the above tests contact the friendly Arasains sales staff or fill in the enquiry form.
For more questions ask Dr Lee
Caution : not all question can or will be answered