SALMONELLA

Salmonella

Salmonella refers to a family of bacteria that causes the most frequently reported food borne illness. The most common types of Salmonella are the serotype S. typhimurium and S. enteritidis. Salmonella are predominantly motile but are non-spore forming. Many isolates exist in two phases a motile phase 1 and a non-motile phase II. The bacteria is gram negative and rod shaped and causes symptoms in humans like vomiting, diarrhea, and cramps especially to the immuno compromised and elderly. Food products where Salmonella is commonly found are chickens, raw meat, eggs, shell fish and in fruits such as water melon and cantaloupe. There are over 2000 serotypes of salmonella and all strains are pathogenic. In order not to have false negatives, the selected test must include all serovars but not show closely related species. Therefore, selecting a good sensitive test is essential. Salmonella can survive for long periods in the environment and water is usually a reservoir. The bacteria can survive freezing but can be destroyed by heat for 10 mins at 75°C.

Conventional Method - Biolife Italiana

Most subspecies of Salmonella can be readily detected by growing them in media containing ferrous sulphate and detecting the production of hydrogen sulphide. However, some species may be atypical. The standard gold method for detecting Salmonella in the lab according to BAM is as follows: Enrichment of 25 g food sample into 225 ml of non-selective enrichment broth, then transferred to RV medium and TT broth, then streaking on BSA, XLD and HE. Arasains supplies the detection media for Salmonella from Biolife.

Lateral Flow – RapidChek^® Salmonella

The RapidChek^® SELECT™ Salmonella test system combines the patented primary phage media with a highly productive secondary enrichment and a latest generation lateral flow device with a proprietary antibody mix to enable fast, accurate and simple Salmonella detection. Time to result: 22 - 30 hours (processed foods and environmental samples), 32 - 44 hours (raw foods).

Benefit:

Fast & simple procedure
Reliable results
Easy resource management
Approved by AOAC RI

Lateral Flow – VIP^® Gold Salmonella

Merck Biocontrol Indonesia offer Salmonella detection using the VIP gold lateral flow strips. The new VIP Gold merges advanced particle technology, highly specific antibodies and an innovative multi-test format to provide increased lab efficiencies and rapid, accurate detection of food-borne pathogens. Easy one step enrichments eliminate sample transfers and reduce labor. Simple assay protocol requires minimal hands-on time. No additional capital equipment such as readers, washers etc. Approved by AOAC OMA.

Benefit:

Fast presumptive screening and easy detection after enrichment steps
Direct application of enrichment sample
Single result format
No skills and no automation required
Robust housing format for better handling

ELISA – TRANSIA™ PLATE Salmonella

Merck Biocontrol Indonesia also provide TRANSIA^™PLATE ELISA technology for detecting Salmonella. It is a robust, flexible test for the Salmonella genus in food. It is designed to work with a simplified, two-step enrichment procedure without the need for M broth to stimulate flagella growth. TRANSIA ELISA technology is based on a three-step, sandwich, immunoenzymatic reaction. The sold support of the reaction is a microtiter plate coated with antibodies targeted against unique lipopolysaccharides (LPS).

Benefit:

Accurate and reliable
High throughput efficiency – 96 well microtiter plate format
Increase Productivity with Automation

R-Biopharm Indonesia Technology – SureFast^® Salmonella ONE

Salmonella spp. detection with real-time R-Biopharm Indonesia. Two in one – Salmonella test kit with included DNA preparation. The kit is intended to be used for the fast and simple isolation and detection of Salmonella DNA from enrichment cultures. Each reaction contains an internal amplification control (IAC).

Benefit:

Integrated DNA preparation
Use the DNA directly for qPCR
No centrifugation/automation needed
Open system for all common qPCR thermocyclers
FAM and VIC/HEX for internal amplification control

PCR Technology – Assurance^® GDS Salmonella

The world is changing. The need for speed and accuracy has intensified. Maintaining the status quo means falling behind. Fortunately, the world of science is changing as well. The Assurance GDS platform is introduced. Based on the latest advancements in molecular technology and food microbiology, Assurance GDS offers faster results and increased accuracy to meet today’s food and environmental testing challenges. The system uses multiple layers of specificity including pick pen for removing matrix interference, highly specific primers and a patented MGB eclipse probe. GDS assays have AOAC, AFNOR validation. Results are available in 24 hours.

Benefit: